Experimentation is at the heart of progress in biology. It enables working hypothesis to be put to the test. Thanks to cycles of hypothesis verification and refinement, predictive models can be developped despite the complexity of the subjects studied: human beings. This course trains future engineer to appreciate the potential and limits of biology experimentation.
This experimental module is an extension of the molecular biology and genetic information (BIO452-P1) and cellular biology and development (BIO451-P2). It also serves as an illustration for the ecology and biodiversity (BIO431) and biology and human pathology (BIO432 - P3).
Depending on the number of students registred, the following topics may be opened:
Three sessions are scheduled over the course of the year: (10 sessions of 6 hours), see Modals booklet X17
- Bioluminescence: two examples: the Jellyfish and the Firefly
Some species have the ability to emit light. This phenomenon, known as bioluminescence, is used by animals as a means of sexual attraction or defense. In this modal, we will analyze how bioluminescence system works, and then, through genetic manipulation, we will adapt this system and make it a powerful tool for biology.
Antibodies: from immune defense to clinical diagnosis:
The goal of this topic is to characterize biochimical proprerities of antibodies and illustrate with various examples the use of antibodies in research and medical diagnosis.
- Intracellular imagery:
The aim of this modal is to observe the various components of the cytoskeleton (microtubule and actin filaments) inside human cells. It provides an opportunity to introduce multiple cell biology techniques (human cell culture, immunocytochemistry, transfection), in microscopy and digital modeling.
- Cloning:
In this experimental module, we will clone a eukaryotic gene into a bacterium, and we will induce its expression and purify the resulting protein. Finally, an enzymbe-based test will be used to functionally characterize the purified protein.
Caution:
With reservation:
*When registering, students can choose experiment A or B. For the choice A (P1 or P3), they can't be guaranteed a specific topic. The dispersal of students is based on supply and demand
Evaluation : Written report and oral defense
Course language: French
- Teaching coordinator: Bouzigues Cédric
- Teaching coordinator: Chessel Anatole
- Teaching coordinator: Lestini Roxane
- Teaching coordinator: Mechulam Yves